![]() ![]() Sequencing of several new strains are ongoing in our laboratory. With the advent of next-generation sequencing (NGS) platforms, so far 37 Cp genomes have been completely sequenced of which Cp1002 is the first sequenced genome. However, so far there is no proper diagnosis method or effective treatment available for Cp infection. It is also capable to cause diseases in cattle and humans. Cp is the etiological agent of the caseous lymphadenitis (CLA) disease, that affects mainly sheep and goat causing huge economic losses by affecting meet and wool production. ![]() Genomic plasticity analysis using 15 Cp strains demonstrates that the group of strains belonging to the ovis biovar are highly similar. Biovar equi is characterized by its capacity to nitrate-reductase production, while the biovar ovis, cannot. Cp can be classified into two biovars: equi and ovis. CP012837.Ĭorynebacterium pseudotuberculosis ( Cp) is a Gram-positive, pleomorphic, facultative intracellular pathogenic bacteria that belongs to the group Corynebacterium, Mycobacterium, Nocardia and Rhodococcus (CMNR). pseudotuberculosis was deposited in GenBank under the accession no. ![]() In conclusion, the results showed by WGM could be used to detect mismatches in assemblies, providing genomic maps with high resolution and allow assemblies with more accuracy and completeness. A specific analysis using BLAST and NR database shows that the inversion occurs between two homology RNA ribosomal regions. After the new assembly process, using WGM as scaffolder, we detected a large inversion with size bigger than one-half of genome. A restriction map was constructed, using the technique of WGM with the enzyme KpnI. The sequencing was performed using the platform Ion PGM and fragment library (200 bp kit). Cp1002 was the first strain of this species sequenced in Brazil, and its genome has been used as model for several studies in silico of caseous lymphadenitis disease. In this work, we present the resequencing of Corynebacterium pseudotuberculosis strain 1002 (Cp1002). In addition, the improving of techniques for construction of high accuracy genomic maps, for example, Whole-genome mapping (WGM) (OpGen Inc), have allow high-resolution assembly that can detect large rearrangements. These new discover have been possible due to the evolution of the Next-Generation Sequencing platforms, which have provided sequencing with accuracy and reduced costs. Error probabilities.Studies have detected mis-assemblies in genomes of the species Corynebacterium pseudotuberculosis. Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred.Koch CM, Chiu SF, Akbarpour M, Bharat A, Ridge KM, Bartom ET, Winter DR (2018) A Beginner’s guide to analysis of RNA sequencing data.Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-seq.Byron SA, Van Keuren-Jensen KR, Engelthaler DM, Carpten JD, Craig DW (2016) Translating RNA sequencing into clinical diagnostics: opportunities and challenges.Ozsolak F, Milos PM (2011) RNA sequencing: advances, challenges and opportunities.Royce TE, Rozowsky JS, Gerstein MB (2007) Toward a universal microarray: prediction of gene expression through nearest-neighbor probe sequence identification.Okoniewski MJ, Miller CJ (2006) Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations.van Hal NL, Vorst O, van Houwelingen AM, Kok EJ, Peijnenburg A, Aharoni A, van Tunen AJ, Keijer J (2000) The application of DNA microarrays in gene expression analysis.Wang Z, Gerstein M, Snyder M (2009) RNA-seq: a revolutionary tool for transcriptomics. ![]()
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